By Y. Asano (auth.), Professor Dr. Y. P. S. Bajaj (eds.)
In continuation of Volumes eight and nine (1989) on in vitro manipulation of plant protoplasts, this new quantity bargains with the regeneration of vegetation from protoplasts and genetic transformation in a number of species of Agrostis, Arabidopsis, Atropa, Brassica, Catharanthus, Datura, Cucumis, Daucus, Digitalis, Duboisia, Eustoma, Festuca, Helianthus, Hordeum, Kalanchoe, Linum, Lobelia, Lolium, Lotus, Lycium, Lycopersicum,Mentha, Nicotiana, Pelargonium, Pisum, Pyrus, Salvia, Scopolia, and Solanum.These reviews replicate the some distance achieving implications of protoplast technologyin genetic engineering of vegetation. they're of designated curiosity to researchers within the box of plant tissue tradition, molecular biology, genetic engineering, and plant breeding.
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Oleracea and B. nigra. Z Pflanzenzuecht 82: 354-360 Schenck HR, Hoffmann F (1984) Isolation and culture of protoplasts: Brassica. In: Vasil IK (ed) Cell culture and somatic cell genetics of plants, vol 1. Academic Press, New York, pp 356-369 Schenck HR, Robbelen G (1982) Somatic hybrids by fusion of pro toplasts from Brassica oleracea and B. campestris. Z Pftanzenzuecht 82:354-360 Shillito RD, Paszkowski SJ, Muller M, Potrykus I (1985) High efficiency direct gene transfer to plants. ) In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 23.
1. Six- week-old white-flowered Brassica alboglabra plant I Institute of Molecular and Cell Biology, National University of Singapore, 10 Kent Ridge Crescent, Singapore 0511, Republic of Singapore. (Present address: Department of Botany, National University of Singapore, Kent Ridge, Singapore 0511) Biotechnology in Agriculture and Forestry, Vol. 22 Plant Protoplasts and Genetic Engineering III (ed. S. Bajaj) © Springer. Verlag Berlin Heidelberg 1993 Regeneration of Plants from Protoplasts of Brassica alboglabra Bailey 23 somatic hybridization or cell transformation, and considerable progress has been made in some species including B.
These include liquid culture (Coutts and Wood 1977; Trulson and Shahin 1986), agarose embedded (in Regeneration of Plants from Protoplasts of Cucumis sativus L. 6% SeaPlaque agarose) followed by hanging-drop culture (Orcyzk and Malepszy 1985), agarose bead culture (Dons and Bouwer 1986) or agarose disc (droplet) culture (Colijn-Hooymans et al. 1988a, b). The culture media utilized were those described by Harada (1973), Durand et al. (1973), Kao (1977), and Murashige and Skoog (1962) (Table 1). Modified MS medium (half-strength macro elements, full-strength minor elements) has been frequently and successfully used in recent studies (Colijn-Hooymans et al.