By Y. P. S. Bajaj (auth.), Professor Dr. Y. P. S. Bajaj (eds.)
Isolated protoplasts are a different software for genetic manipulation of crops. because the discovery of a mode for the enzymatic isolation of pro-· toplasts by means of Professor E. C. Cocking in 1960, large development has been made during this very attention-grabbing sector of analysis. i've got witnessed the fight within the 1960's and early 1970's, whilst acquiring a fresh prepara tion of protoplasts was once thought of an fulfillment. I additionally shared the pioneering pleasure and exuberance during this box in the course of the second Inter nationwide Congress of Plant Tissue tradition held at Strasbourg in 1970, the place Dr. I. Thkebe of Japan awarded his paintings at the induction of department in tobacco protoplasts. This used to be by means of my participation in 3 foreign meetings dedicated to plant protoplasts held in 1972 in Salamanca (Spain) and Versailles (France), after which in 1975 in Nottingham (England). the passion proven through plant scientists at those conferences used to be plentiful facts of the intense way forward for protoplast know-how, and it turned glaring that protoplasts might playa significant function in plant biotechnology, in particular in genetic engineering. considering that then we now have by no means seemed again, and now the tools for isolation, fusion, and tradition, in addition to regeneration of somatic hybrids, became regimen laboratory systems for many plant species. at the moment the focal point is on cereal and tree protoplasts. so that it will result in any genetic manipulation via fusion, in company of DNA, and transformation, the regeneration of the whole plant via manipulation of protoplasts is a prerequisite.
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Extra resources for Plant Protoplasts and Genetic Engineering I
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BioI Cell 58:173 -178 Carlberg I, Glimelius K, Eriksson T (1984) Nuclear DNA content during the initiation of callus formation from isolated protoplasts of Solanum tuberosum L. Plant Sci Lett 35:225-230 Carlson PS (1970) Induction and isolation of auxotrophic mutants in somatic cultures of Nicotiana tabacum. Science 168:487 - 489 Carlson PS (1973 a) The use of protoplasts for genetic research. Proc Natl Acad Sci USA 70:598-602 Carlson PS (1973 b) Methionine sulfoximine-resistant mutants of tobacco.
Plant Sci 50:161-169 De Vries SE, Thmpelaar MJ (1987) Electrofusion and analysis of potato somatic hybrids. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry 3. Potato. Springer, Berlin Heidelberg New York Thkyo, pp 211-222 Dons JJM, Bouwer R (1986) Improving the culture of cucumber protoplasts by using an agarose-disc procedure. In: Nuclear techniques and in vitro culture for plant improvement. IAEA, Vienna, pp 496-504 Dowgert MF, Steponkus PL (1983) Effect of cold acclimation on intracellular ice formation in isolated protoplasts.
The yield from petunia leaves, however, was not affected by this removal. Xylanase was not an essential, but a quite significant enzyme for the isolation of protoplasts from oat leaves. Ishii (1984) demonstrated that both pectin lyase and xylanase could macerate grass leaves. A mixture of the two enzymes brought about more rapid isolation of single cells than did either of the two enzymes alone, indicating a synergistic effect. In dicots and Liliaceae tissues there was no synergistic effect between pectin lyase and polygalacturonase in tissue maceration (Ishii 1976), but the maceration of these tissues by pectinases was greatly stimulated by the addition of an enzyme-like stimulating factor produced by Aspergillus japonicus (Ishii and Kiho 1976).