By Sean Wilsey
Content material: review of proteomics -- Proteomic instruments for research of mobile dynamics -- Dynamics of useful mobile equipment : from statics to dynamics in proteomic biology
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Principle for Experimental Specification of a Modification 23 Lys Ubiquitination Ubiquitin (polypeptide) 140 159 42 Ͼ1000 140 N terminus CH3CO 158 Arg/Lys N terminus 157 Arg Acetylation Catalyzed by methyltransferase (continued) The proteins are purified by immunoaffinity chromatography with anti-ubiquitin antibody under denaturing or native conditions. They are then digested with trypsin, and the resulting peptides were analyzed by 2DLC-MS/MS. 1 Da) dipeptide. Top–down MS/bottom–up MS/MS. Direct LC-MS/MS identification of isolated Golgi fraction.
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