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L I 2 1 0 cells were treated for 1 hr with 20 μΑί cisplatin or 20 μΜ L-PAM. Survival (upper panels): o , at intervals following removal of drug, cells were exposed to 100 mM thiourea for 1 hr; · , no thiourea. Interstrand crosslinking (lower panels): ±, immediately following removal o f cisplatin or L-PAM, cells were exposed to 100 mM thiourea for 1 hr and then incubated in drug-free medium for the indicated time; o, same except that the thiourea exposure occurred during the last 1 hr before harvest; · , no thiourea.
Hematol Oncol 7, 349-373. Woodman, R. , Sirica, A. , and Venditti, J. M. ( 1 9 7 3 ) . Chemother. 18,169-183. Yager, J. , and Miller, J. A. ( 1 9 7 8 ) . Cancer Res. 38, 4 3 8 5 4 3 9 4 . Young, R. , and Fischer, G. A. ( 1 9 6 8 ) . Biochem. Biophys. Res. Comm. 32, 2 3 - 2 9 . Zwelling, L. , Kohn, K. , Ross, W. , Ewig, R. A. , and Anderson, T. ( 1 9 7 8 ) . Cancer Res. 38,1762-1768. Zwelling, L. , and K o h n , K. W. ( 1 9 7 9 ) . Cancer Res. 39, 3 6 5 - 3 6 9 . Cisplatin Chapter 5 REPAIR OF cis-PLATINUM (II) DIAMMINE DICHLORIDE-INDUCED DNA DAMAGE AND CELL SENSITIVITY John J.
II. MATERIALS AND METHODS A. Cell Line Cells used in this investigation (designated LoVo) are from a carcinoembryonic antigen-producing colon adenocarcinoma cell line. Biological properties and growth kinetics characteristics have been published elsewhere (Drewinko et al, 1976; Drewinko et al, 1978). Monolayer cultures are maintained in Ham's F-10 medium supplemented with 20% fetal calf serum, vitamins, glut amine, and anti biotics. 5% trypsin (5 min at 37°). , 1978). Β. Drugs Cis-DDV manufactured by Bristol Laboratories, Syracuse, New York and ara-C, manufactured by Upjohn Laboratories, Kalamazoo, Michigan, were ob tained from the Division of Cancer Treatment, National Cancer Institute.