By Itzahak Kahane, Amiram Adoni
This compendium is the results of the FEMS Workshop on "Rapid prognosis of Mycoplasmas" which I prepared and which happened in Jerusalem, Israel, August 11-23, 1991. the 1st week's classes have been held at a hotel at the outskirts of Jerusalem and consisted of lectures and discussions. This half was once modelled alongside the strains of the Gordon convention within the united states, i.e., in an intimate atmo sphere within which all people may well combine and alternate rules, and was once very benefi cial. approximately a hundred scientists from all over the world attended the 1st week. Dur ing the 1st week, the biology, molecular biology and pathophysiology of myco plasmas, in addition to the entire major diagnostic equipment have been lined, together with either traditional and the more recent applied sciences. The consultation on mycoplasmas within the human urogenital tracts was once held at the side of the Israel Society for the research and Prevention of Sexually Transmitted ailment. the second one week was once a laboratory consultation and was once held on the Hebrew University-Hadassah scientific university campus in Ein Karem, Jerusalem. All ex periments have been carried out by means of eminent experts of their box. The lab consultation had 36 members from 19 international locations who used the main smooth strategies for the prognosis of mycoplasmas in drugs, veterinary medication and agri tradition. The efficacy of a number of advertisement kits have been additionally validated at present. i would like to back thank all people who helped and supported this paintings store, in addition to the authors of a few of the chapters.
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Renaudin and J. M. Bove, unpublished). As shown in Figure 3, more than 3/4 of the S. citri RBA2 HP genome contained SpVI-RBA2 B DNA sequences. Only one region, about 20% of the genome, contained no viral DNA sequences (Fig. 3). Similar results were obtained with several S. citri pVl viruses, including SpVl RBA2 Band SpVl-7B. These viral strains show strong cross hybridization among themselves. 3 kb of SpVI-RBA2 B DNA. To our surprise, there was practically no cross-hybridization between the DNAs of viral strains RBA2 Band S102 and, furthermore, only one small region of the S.
Citri virus SpVl- R8A2 B can be used as a vector to insert and amplify genes into cells of S. , 1991). The gene for chloramphenicol acetyl transferase (CAT) and responsible for resistance to chloramphenicol was used as the model gene. Indeed, it was possible to insert the CAT gene into the intergenic region 13 at the single MboI site of the viral RF, transfect the spiroplasma cells by electroporation with the recombinant RF, get transcription and translation of the CAT gene in the spiroplasmal host, and recover Sp VI viruses containing the CAT sequences as an insert (Sp VI-CA T).
Citri genome is even more complex than was thought. , I990a). , I990b; P. Aullo, J. Renaudin and J. Bove, unpub- 26 lished). To that purpose, a genomic DNA library of s. citri R8A2 HP DNA has been established in a phage A. derived vector. Several recombinant A. clones hybridizing with SpVI-R8A2 HP DNA have been detected. One clone (LtO) has been further analyzed. The DNA of clone LtO was shown to contain a fulllength SpVl genome; the junctions between viral DNA and spiroplasmal chromosomal DNA have been sequenced, thus identifying the side where the circular viral RF DNA has to be cut (linearization) for insertion as a linear viral DNA into the S.