By Jan Albert, Eva Maria Fenyö (auth.), Professor Yechiel Becker, Professor Gholamreza Darai (eds.)
The foundation for the potent therapy and remedy of a sufferer is the quick analysis of the affliction and its causative agent, that's in line with the research of the medical signs coupled with laboratory assessments. even if speedy develop ments were made within the laboratory prognosis of virus illnesses, the neces sary isolation of the causative virus from the medical specimens is a comparatively lengthy method. Viruses which combine into the mobile DNA (such as human immunodeficiency virus, HIV -1, or hepatitis B virus) are tough to spot by means of molecular suggestions, whereas viruses which exist within the scientific fabric in low concentrations are much more daunting to establish. lately, the applying of the polymerase chain response (peR) approach built by way of okay. D. Mullis and unique within the learn through Saiki et al. (1985) ended in a revolution in virus prognosis. The in line with method used to be quickly utilized to the prognosis of viruses in medical fabric. quantity 1 of Frontiers of Virology offers new info at the advan tages of using the consistent with for the analysis of many human disease-causing viruses, in addition to on a few issues of its use.
Read Online or Download Diagnosis of Human Viruses by Polymerase Chain Reaction Technology PDF
Similar diagnosis books
This pocket consultant presents day by day aid for dwelling with COPD through providing instant connection with clinical info and sensible techniques for facing the actual, emotional, affliction. Mark Jenkins specializes in how you can keep optimum actual and psychological overall healthiness and lays out useful administration ideas for residing with COPD
Fundamental Basis of Irisdiagnosis: Interpretation and Medication
Booklet by means of Kriege, Theodor
Laboratory Tests and Diagnostic Procedures
Glance no extra for speedy, entire solutions to questions comparable to which laboratory checks to reserve or what the implications could suggest. Laboratory checks And Diagnostic techniques, fifth variation covers extra exams than the other reference of its sort, with over 900 lab checks and diagnostic systems in all. partly I, you will discover a different, alphabetical record of 1000's of ailments, stipulations, and signs, together with the exams and approaches most typically used to substantiate or rule out a suspected analysis.
CMR and MDCT in Cardiac Masses: From Acquisition Protocols to Diagnosis
This booklet, specific in focusing in particular on cardiac plenty, is the results of cooperation between a few groups of radiologists operating less than the aegis of the French Society of Cardiovascular Imaging (SFICV). Its target is to one) evaluation different CMR sequences and CT acquisition protocols used to discover cardiac plenty, 2) to illustrate the various CMR and MDCT positive aspects of cardiac lots.
Additional resources for Diagnosis of Human Viruses by Polymerase Chain Reaction Technology
Example text
1988). To try to validate the use of this new technology for the early diagnosis of HIV infection in children, my approach was first to compare its sensitivity and specificity with the other methods of HIV -1 detection and then to evaluate its sensitivity during the first weeks of life. I report on a retrospective study conducted in Boston in 1988-1989 and a prospective study conducted in Paris in 1990. Comparative Study of Virus Isolation, PCR, and Antigen Detection Material and Methods In this study (Krivine et al.
1985; Osame et al. 1986). Recently, the association of HTLV-I infection with other types of clinical manifestations such as alveolitis (Sugimoto et al. 1989), Sjogren's syndrome (Vern ant et al. 1988), polymyositis (Wiley et al. 1989), and chronic inflammatory arthropathy (Nishioka et al. 1989) has been suggested. The main routes of transmission of HTLV-I described to date are blood transfusion (Okochi et al. 1984), breast milk, and sexual contact from male to female via semen (Komuro et al. 1983; Tajima et al.
1989; Blanche et al. 1989). Since zidovudine therapy has been shown to reduce the level of p24 antigenemia and the neurological impairment of infected children (McKinney et al. 1991), it is crucial to be able to make the diagnosis of perinatal HIV-1 infection as early as possible. Diagnosis of HIV -1 infection in children aged 15 months or older is based, like in adults, on the detection of HIV-specific antibodies in the serum. Since all children born to HIV seropositive mothers carry passively transmitted maternal antibodies at birth, alternative methods of diagnosis have been developed that directly address the presence of virus or proviral DNA in the sample; these methods include virus isolation from peripheral blood mononuclear cells (PBMC) (Jackson et al.