By C. F. A. Culling
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Zenker's fluid is a good routine fixative giving fairly rapid and even penetration. Following its use, tissues must be washed in running water overnight to remove the excess dichromate (page 33), and mercuric chloride pigment must be removed with iodine (page 33). Fixation is usually complete in 12 hours. Small pieces, not thicker than 3 mm are fixed in 2-3 hours. 5 g 1g to 100 ml 5 ml This fixative is stock Zenker mixture to which formalin is added instead of acetic acid. It is irrational in that it contains potassium dichromate which is an oxidizing agent, and formaldehyde which is a reducing agent, but it is an excellent micro-anatomical fixative.
Both of these fixatives are most usefully employed as secondary fixatives (page 45). Care should be taken to observe safety rules in disposing of mercury-containing solutions. 5 g 1g to 100 ml 5 ml This solution does not keep after the acetic acid has been added, but without acetic acid (Zenker's stock fluid) it keeps well and has the advantage that either acetic acid or formalin may be added immediately before use (see below). Zenker's fluid is a good routine fixative giving fairly rapid and even penetration.
There are suggestions that the use of mercuric chloride may enhance the demonstration of certain peptides by immunocytochemistry (Bosman et aU 1977). Precautions must be taken with the disposal of mercury-containing fixatives to avoid environmental pollution. Potassium dichromate (K 2 Cr 2 0 7 ) Potassium dichromate has a binding effect on protein similar to that of formalin, giving fixation of the cytoplasm without precipitation. It is thought that this is because chromium ions form complexes with water which combine with the reactive sites on adjacent protein chains.